Detection of macrophages in human and rat heart using a single antibody variant
نویسندگان
چکیده
BACKGROUND: Resident macrophages of the myocardium are necessary for normal development and functioning cardiovascular system play an important role in pathogenesis a number diseases.
 AIM: To develop universal methodological approach immunohistochemical identification human rat myocardium.
 MATERIAL AND METHODS: For study, samples people both sexes aged from 28 to 52 years (n=7) Wistar female rats (at age 15 months, (n=5)) were used. The work was performed using staining method.
 RESULTS: possibility polyclonal antibodies against calcium-binding protein Iba1, which is well-known microglial marker marker, detection shown. An optimal drug processing protocol developed, use makes it possible equally effectively detect only one antibody variant.
 CONCLUSION: A has been proposed myocardium, can be successfully used scientific clinical diagnostic studies.
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ژورنال
عنوان ژورنال: Morfologii?a? (Saint Petersburg, Russia)
سال: 2023
ISSN: ['1026-3543']
DOI: https://doi.org/10.17816/morph.200003